Feifei Dong

South-Central Minzu University, China

Title: Study on the Effects and Mechanism of Action of ?-ionone on the Behavior of Sleep-deprived Mice

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Biography

Feifei Dong, a graduate student in the Biomedical Engineering College at South-Central Minzu University. Her current research focuses on investigating the effects of essential oils derived from traditional Chinese medicine on sleep disorders and emotional illnesses. This work aims to explore potential alternative therapies and their underlying mechanisms for improving mental well-being.

Abstract

Sleep is a crucial safeguard for maintaining normal physiological processes in humans, playing significant roles in promoting growth and development, as well as forming and consolidating memories. With the acceleration of people's pace of life, sleep deprivation (SD) caused by insufficient sleep has gradually become a prevalent phenomenon among the population, sleep deprivation has severely compromised people's physical health. To investigate the behavioral effects of ?-ionone on sleep-deprived mice, this study primarily evaluated sleep indicators through collaborative sleep experiments, assessed depressive-like behaviors through the tail suspension test and forced swim test, evaluated anxiety-like behaviors through the open field test and elevated plus maze test, and assessed learning and memory abilities through the Y-maze test. This study employed ELISA experiments to examine the impact of ?-ionone on inflammatory factors and neurotransmitters in the serum of sleep deprived mice, and utilized Western Blot to detect changes in the expression levels of proteins involved in related pathway. Behavioral studies have shown that ?-ionone can alleviate anxiety and depressive-like behaviors induced by sleep deprivation, as well as enhance learning and memory abilities in mice. The ELISA results indicated that ?-ionone could reduce the levels of inflammatory factors IL6,IL1?,TNF? and increase the levels of GABA and BDNF. Western Blot results indicate that ? -ionone can alleviate behavioral abnormalities induced by sleep deprivation by regulating the phosphorylation levels of PI3K/AKT/GSK3 ? .In summary, we conclude that ? -ionone improve anxiety and depressive-like behaviors caused by sleep deprivation, as well as enhance learning and memory abilities by regulating the PI3K/AKT/GSK3? pathway.

Keywords

?-ionone; sleep deprivation; ethological experiment; GABA; BDNF; PI3K/AKT/GSK3? pathway